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Figure 10


Fig. 10. Twf1 promotes actin filament disassembly in vivo. (A) Wild-type (WT), twf1{Delta} and cof1-22 yeast cells were grown in parallel to OD600=0.3, then treated with 50 µM Lat-A. Samples of cells were removed 0 and 5 minutes after addition of Lat-A, fixed in 5% formaldehyde, and stained for filamentous actin using Alexa Fluor 568-congugated phalloidin. Cells were visualized by fluorescence light microscopy and scored for the presence of visible actin patches and/or cables. >200 cells were counted for each data point, and similar results were obtained in two independent experiments. Treatment of cells with control buffer caused no loss of actin structures (not shown). (B) Representative wild-type (WT), twf1{Delta} and cof1-22 cells stained with Alexa Fluor 568-congugated phalloidin 0 minutes and 5 minutes after treatment with Lat-A.





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