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Fig. 2. Concentration-dependent effects of Twf1 on rates of dilution-induced actin filament disassembly. (A) Time course of actin filament fluorescence upon dilution (as in Fig. 1) in the presence of a range of concentrations of Twf1 at pH 5.0. (B) Concentration-dependent effects of Twf1 on the initial rate of decrease of actin filament fluorescence at different pH values. Rates were obtained from fluorescence curves in A and data not shown. (C) Comparison of Twf1 and Cof1 effects on the initial rate of actin filament fluorescence decrease at pH 5.0. Rates were obtained from fluorescence curves in A and data not shown. F.A.U., fluorescence arbitrary units.