spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Residues 1-33 of NOS2 are not part of the catalytic core of NOS2. (A) Absorbance spectrum of the recombinant NOS2 ({Delta}33) expressed in E. coli. The contribution of the heme prosthetic group is centered around 390 nm whereas the contribution of the FAD and FMN flavins is centered around 450 nm. The insert shows a Coomassie Blue-stained gel with molecular weight markers (M), boiled (B) NOS2 and unboiled (U) NOS2, where the SDS-resistant dimers (D) can be partially observed as distinct form the monomers (M). Approximately 2 µg of purified protein were loaded per lane. Panel B show the catalytic activity of the recombinant NOS2({Delta}33) (delta-33) protein compared with the wild-type NOS2 (wt).





Right arrow Return to article