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Figure 9


Fig. 9. The non-palmitoylated C3S is able to form dimers. COS7 cells were cotransfected with full-length NOS2 with either the wild-type or the C3S (1-94)-NOS2-GFP chimera. Antibodies that recognise the C-terminus of NOS2 or the GFP tag were used independently to immunoprecipitate the complexes. The immunodetection (western blot) was performed using both anti-GFP antibodies (upper panel) and anti-NOS2 antibodies (bottom panel). The (1-94) NOS2-GFP chimeras appear at 42 kDa whereas the NOS2 appears at 133 kDa.





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