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Fig. 7. Src and Ras mediate TRAF6 activation of PI 3-kinase. A PH-Akt-GFP expression vector reporter was co-transfected along with indicated plasmids into HEK293 cells. Src- and Ras-specific inhibitors were each added, as indicated above each panel, to a final concentration of 500 nM into individual wells at 6 hours. Images were taken 24 hours after transfection.