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Fig. 8. TRAF6 is relocalized when co-expressed with either an upstream or downstream activator. (A) Subcellular localization of TRAF6-YFP co-transfected with various expression vectors encoding non-labeled activator proteins. 70 ng total DNA (20 ng of TRAF6-YFP and 50 ng of expression vector, as indicated) were co-transfected into HEK293 cells. `Control' represents cells co-transfected with 20 ng TRAF6-YFP and 50 ng of empty expression vector. Src, MyD88 and IRAK2 are wild-type expression vectors. Src(1-142) and Src(1-82) expression vectors contain the regions indicated in Fig. 4. (B) NF-B activity resulting from co-transfection in HEK293 cells with expression vectors, as indicated, and the luciferase reporter described in Fig. 5. The amount of transfected vectors is the same as in Fig. 8A. A total of 70 ng of DNA was used for each transfection, including the empty `Vector' control. (C) PH-Akt-GFP expression vector reporter was co-transfected together with cDNA vectors encoding proteins, as indicated above each panel, into HEK293 cells.

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