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Figure 1


Fig. 1. NFATc1-GFP has a predominantly nuclear localization in most slow muscle fibers and a predominantly cytoplasmic localization in most fast fibers. (A-D) Longitudinal (A,B) and transversal (C,D) sections of mouse soleus (A,C) and tibialis anterior (TA) muscles (B,D) transfected with plasmids coding for NFATc1-GFP fusion protein. Notice the nuclear localization of NFATc1-GFP in soleus (A,C) and the presence of multiple foci of GFP fluorescence when nuclei are examined at higher magnification (A, inset). By contrast, NFATc1-GFP is either homogeneously distributed in the nuclei and cytoplasm of transfected TA muscle fibers (B,D) or some nuclear profiles are GFP-negative in this muscle, as shown by DAPI staining (D, inset). (E-F) Transverse sections of soleus (E) and TA (F) muscles transfected with plasmids coding for a constitutively active flag-tagged mutant of NFATc1 (caNFATc1) and analyzed with anti-flag antibodies (left panels) and nuclear DAPI staining (central panels). Images were merged to demonstrate colocalization (right panels). Bars, 30 µm (A-F); 5 µm (inset in A); 10 µm (inset in D).





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