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Figure 5


Fig. 5. Basal CNT2 expression and activity in intestinal and liver cells. (A) Reverse transcriptase (RT)-PCR was performed on RNA from intestinal mucosa (I), IEC-6 cells, liver (L), FAO cells and rat liver parenchymal cells (Hep). (B) CNT2 protein expression was analyzed in crude extracts (H) and total membrane (M) fractions from IEC-6 cells, FAO cells and rat hepatocytes (Hep) by western blot. (C) CNT2-mediated uptake (open bars) was measured, as indicated in the Materials and Methods, using 1 µM adenosine as a substrate and 1 minute incubation time. Na+-independent adenosine uptake (solid bars) corresponds to the sum of ENT1- and ENT2-related transport activities, plus putative binding. Results are the mean ± s.e.m. of four experiments.





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