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Figure 7


Fig. 7. Adenosine activates AMPK and phosphorylates its downstream target in the hepatoma cell line FAO and primary culture of rat hepatocytes. (A) FAO cells (Fao) were treated with either 10 µM adenosine (Ado) or 500 µM AICAR (Aicar) for 30 minutes and 60 minutes, respectively. A representative western blot of the phosphorylated form of ACC (P-ACC) and densitometric analysis of four independent experiments are shown. (B) Primary cultures of rat hepatocytes were incubated in the presence of 10 µM adenosine and protein extracts were analyzed at the indicated times for ACC phosphorylation. A western blot and the corresponding densitometric analysis of three independent experiments are shown. ß-actin is used as a loading control. The statistical significance was assessed by Student's t-test: A: ***P<0.001; B: **P<0.01. Ctrl, control.





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