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Figure 3


Fig. 3. (A) 3' UTR sequence of {alpha}2 mRNA. This sequence was obtained by direct sequencing and by cloning and sequencing of two overlapping PCR fragments. The two oligonucleotide pairs for PCR are indicated in bold and underlined, and are labelled as F1, R1 and F2, R2. ARE sequences are indicted in bold capital letters. (B) An agarose gel showing the PCR products obtained with the different oligonucleotide pairs. Lanes 1, 3 and 5 correspond to the PCR products obtained after RT. Lanes 2, 4 and 6 show that no amplification was observed in the absence of RT; dT, oligodT.





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