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Figure 6


Fig. 6. NMDA stimulates the NO-cGMP pathway in granule cells. (A) NMDA increases intracellular Ca2+ levels, as measured with the fluorimetric probe FLUO-4AM. (B) NMDA increases intracellular NO as measured with the fluorimetric probe DAF-FM. (C-E) NMDA also stimulates (C) cGMP, (D) VASP phosphorylation and (E) CREB phosphorylation. For immunocytochemistry, cells were incubated in the presence of 0.5 mM IBMX for 30 minutes and then fixed or stimulated with 100 µM NMDA for10 minutes before processing as described in Materials and Methods.. For phosphorylation studies, cells were incubated with 100 µM NMDA for 15 minutes in the presence or absence of 20 µM Rp-8-pCPT-cGMPS added 30 minutes before the stimulation. VASP and CREB phosphorylation was analysed with specific antibodies as described in the Materials and Methods. *P<0.001, significant difference from control values.





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