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Fig. 7. C8-PA-induced scattering requires ERK1/2 and EGFR activities. (A) MDCK cells were incubated overnight with 250 µM C8-PA and 50 µM LY294002, 10 µM UO126, or 10 µM tyrphostin AG 1478 as indicated, and photographed using phase contrast optics. Bar, 50 µm. (B) Time-course for ERK1/2 activation. MDCK cells were treated with 375 µM C8-PA or 10 ng/ml HGF for the indicated periods of time and extracts were prepared and immunoblotted with an antibody against the activated forms of ERK1/2. (C) Activation of the EGFR by C8-PA in MDCK cells. Cells were pre-incubated with 10 µM UO126, 10 µM tyrphostin AG 1478, 50 µM GM6001, and then treated with C8-PA for 15 minutes or 10 ng/ml EGF for 10 minutes. Equal loads in the lanes were verified by staining the blots with Ponceau S (see Materials and Methods). (D) Activation of the EGFR by C8-PA in human corneal epithelial cells. Conditions were as in C. Preincubation with 50 µg/ml CRM 197 or 20 µg/ml antibody was for 6 hours.
