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Fig. 4. Endogenous SH2-Bß interacts with RET in rat-tissue homogenates. Different tissue homogenates from mesencephalon, spinal cord, kidney and muscle were prepared as described in Materials and Methods, and then immunoprecipitated with anti-RET or anti-SH2-Bß antibodies, followed by immunoblotting with anti-SH2-Bß or anti-RET antibodies. Protein expressions in tissues were visualized by immunoblotting with anti-SH2-Bß or anti-RET antibodies.