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Figure 5


Fig. 5. SH2-Bß and RET are colocolized in rat mesencephalic neurons. (A) The tissue slices from rat mesencephalon were first immunostained with anti-SH2-Bß antibodies (green) and then immunostained with anti-RET antibodies (red). The merged images indicate that SH2-Bß and RET can colocolize in red nucleus neurons (yellow) of rat mesencephalon. Bars, 40 µm (top), 20 µm (bottom). (B) Subcellular localization of SH2-Bß in primary cultured rat mesencephalic neurons. The distribution of SH2-Bß (green) is shown by immunofluorescence, its colocalization with either the dendrite marker MAP-2 or the axon marker tau-1 (red) is shown in the merged images (yellow). Bar, 20 µm.





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