spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Cad39 but not Cad40 localises to podosomes. A7r5 cells expressing EGFP-Cad40 (A-D) or EGFP-Cad39 (E-H) fragments of caldesmon were fixed and stained 48 hours after transfection (A,B,E,F) or stimulated with 1 µM PDBu for 30 minutes prior to fixation (C,G). Cells were stained for EGFP (green) or F-actin (red) and an overlay of the two channels is shown. (A,B) Cad40 disrupted stress fibres in 50% of transfected cells (A), while cells expressing lower levels of Cad40 retained stress fibres (B), which did not contain Cad40. (C,D) Cad40 did not translocate to podosomes induced by PDBu. (D) A reconstruction of the x-z profiles of podosomes from cells in C. (E,F) Cad39 was less disruptive to stress fibres, affecting only 20% of the transfected cells (E) and the majority (80%) of the cells showed little disorganisation of stress fibres (F). Unlike Cad40, Cad39 localised to stress fibres. (G,H) Cad39 co-localised to the core of the podosome. (H) The x-z profiles of podosomes from cells in G. Bars, 20 µm in A-C,E-G; 2 µm in D,H.





Right arrow Return to article