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Figure 1


Fig. 1. Yeast two-hybrid analysis reveals an interaction between TC48 and p23/p25. (A) The PJ69-4A yeast strain was co-transformed with TC-PTP (TC48 or TC45) GAL4-DNA-binding-domain fusion constructs and p23 or p25 GAL4-activation-domain fusion constructs obtained in the yeast two hybrid screen. Transformants were grown on plates with (Ade+) or without (Ade-) adenine and also on plates supplemented with ß-galactosidase substrate X-gal (X-gal+). Ade- is the yeast-medium-plate lacking Leu, Trp and Ade, in which activation of the adenine reporter gene is being tested. Ade+ is the yeast-medium-plate lacking Leu and Trp, selecting only for the presence of plasmids to be tested for interaction. (B) Interaction of the C-terminal 66 aa (C-66) of TC-48 with p23 and p25. (C) Schematic representation of TC48 and TC45. The C66-TC48 construct encodes the C-terminal 66 aa of TC48 (from 350-415); aa 1-381 are identical in TC48 and TC45. (C) Control plasmid pGBKT-7; TC45-IP is a positive control for the TC45-interacting protein.





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