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Fig. 2. Interaction of C-terminal sequences in p23 and p25 with TC48. (A) Schematic representation of p23 and p25 and various deletion mutants cloned in yeast vector pAct-II. LD, lumenal domain; CC, coiled-coil domain; TM, transmembrane domain; CT, cytoplasmic tail. (B) Yeast strain PJ69-4A was co-transformed with TC48 (cloned in pGBKT-7) and various mutants of p23 or p25. Transformants were grown on plates with (Ade+) or without adenine (Ade-).