|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Overexpression of p23 and p25 affect localization of TC48. GFP-TC48 was transfected in Cos-1 cells; after 24 hours cells were stained with antibodies that recognize endogenous p23 (A) or p25 (D). Stained cells were examined by confocal microscopy. Cos-1 cells were co-transfected with GFP-TC48 and myc-p23 (B) or myc-p25 (E) and fixed after 12 hours of transfection. Fixed cells were stained with anti-myc mouse monoclonal antibody and Texas-Red-conjugated secondary antibody to visualize p23 and p25 proteins. The images shown are the middle optical sections taken, using the apotome fluorescence microscope. (C) GFP-p23- and rat TC48-expression plasmids were co-transfected in Cos-1 cells. After 24 hours cells were fixed, stained with G11 monoclonal antibody (red) and visualized by confocal microscopy. (F) Cells were transfected with myc-p25SS and GFP-TC48 and processed as in (E) after 12 hours of transfection. Bars, 10 µm.
|
