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Figure 7


Fig. 7. Interaction of C-terminal sequences of TC48 with p23 and p25. (A) Yeast two-hybrid analysis was performed between various N-terminal deletion mutants of TC48 and p23 (pAct-II) or p25 (pAct-II). TC48 is the full-length TC48, whereas C40aa and C66aa are the C-terminal 40 aa and 66 aa of TC48 fused with the DNA-binding domain of Gal4 in pGBKT-7 (bait vector). pGBKT-7 and pAct-II are the empty Gal4-DNA-binding domain and Gal4-activation-domain-containing yeast two-hybrid expression vectors, respectively. (B) Cos-1 cells were co-transfected with GFP-C40 and myc-p23 (upper panels) or GFP-C66 and myc-p25 (lower panels) expression plasmids. After 36 hours cell lysates were prepared and immunoprecipitated with myc antibody or control antibody (C). Western blots of cell lysates and immunoprecipitates (IP) with GFP and myc antibodies are shown. Input is 5% total cell lysate.





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