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Figure 8


Fig. 8. Localization of GFP-C40 and GFP-C66 fusion proteins. Cos-1 cells transfected with vectors expressing GFP-C40 (A) or GFP-C66 (B) were allowed to express exogenous cDNA for 24 hours. Cells were fixed and stained with anti-giantin mouse monoclonal antibody (red) to stain the Golgi complex, and observed by confocal microscopy. Yellow colour indicates colocalization of GFP-C40 or GFP-C66 and Golgi-localized giantin. (C) GFP-C66 and myc-p25 expression plasmids were co-transfected in Cos-1 cells; after 12 hours of transfection the cells were fixed, stained with anti-myc antibody (red) and observed by confocal microscopy. (D) GFP-C40 and myc-p23 expression plasmids were co-transfected in Cos-1 cells; after 12 hours of transfection the cells were fixed, stained with myc antibody (red) and observed by confocal microscopy. Bars, 10 µm.





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