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Figure 2


Fig. 2. LacZ staining and immunofluorescence analyses of the uterus of different genotypes. (A-C) LacZ staining of untreated (A), tamoxifen-treated (B) Cx43fl/fl:SM-CreERT2 and Cx43del/+ (C) uterine tissue was performed according to standard protocols. (D,E) Cryosections of untreated (D), tamoxifen-treated (E) Cx43fl/fl: SM-CreERT2 uterus were analysed by immunofluorescence using antibodies to Cx43 (red) and smooth muscle actin (green). Inserts represent magnified images. CM, circular muscle layer; LM, longitudinal muscle layer; S, stroma. Bar, 50 µm.





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