JCS02897 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. A Xenopus spinal neuron growing for 3 hours in the absence of any drug or EF. The timer represents minutes elapsed. Note the highly motile growth cones, which extend in random directions. Bar, 50 μm.

• Movie 2 -

  Movie 2. A neuron growing in an EF of 150 mV/mm for 3 hours with the cathode at the left and the anode to the right of the image. The growth cones are highly motile and turn towards the cathode within minutes (timer represents minutes since EF initiated). Note that the growth cone to the right maintains its trajectory towards the cathode even when it meets an obstacle (the other neurite). Bar, 25 μm.

• Movie 3 -

  Movie 3. Detail of a growth cone in medium containing the Rac CRIB inhibiting peptide (Rac 17-32). The EF is on from the start. Note that the growth cone has several well-developed, active filopodia but few distinct lamellipodia. The growth cone fails to turn significantly towards the cathode (left). Bar in the first frame is 25 μm.

• Movie 4 -

  Movie 4. Detail of a growth cone in the presence of an EF (cathode left) and the Cdc42 CRIB inhibiting peptide (Cdc42 17-32). The EF is on from the start of the movie. The growth cone has a well-developed and highly motile lamellipodia but does not turn significantly towards the cathode (left). Bar in the first frame is 25 μm.