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Figure 1


Fig. 1. Actin microfilaments are required for growth cone turning towards the cathode. (A) Mean angle turned in 3 hours; lat, latrunculin; n, number of growth cones measured; ns, not significant. *P<0.05; **P<0.005; ***P<0.0001. Black asterisks indicate comparison with `DMSO + EF' and grey asterisks indicate comparison with `DMSO, no EF'. (B) Mean migration rates for the growth cones in A. (C) The frequency of turning towards the cathode (filled bars) and the anode (open bars) for the same population. #P<0.002; ##P<0.001 compared with DMSO + EF. (D) Composite drawings of individual neurons at 3 hours made by superimposing cell bodies at the coloured dot and tracing individual neurite paths. For EF-treated cells, the EF vector is horizontal with the cathode to the left and the anode to the right. Bar, 100 µm, applies to all.





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