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Figure 10


Fig. 10. Inhibition of PARPs impairs nucleolar localization of CTCF-full length, but not CTCF-ZF. UR61 cells were treated for 8 hours with 3-aminobenzamide (ABA) and then transfected with the GFP-CTCF full length (GFP-CTCF; a-c,d-f,g-i), or with the GFP-CTCF-zinc-finger domain (GFP-ZF; j-l). 24 hours post-transfection cells were analyzed by confocal microscopy for green fluorescence (a,d,g,j). Nucleoli were stained with propidium iodide (PI; b), or immunostained for UBF (e). Cells were also pulsed with 5'-FU for 10 minutes (h,k); merged images are also shown (c,f,i,l).





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