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Figure 3


Fig. 3. Nucleolar localization of endogenous CTCF in rat neurons in vivo. (A) Confocal laser microscopy following double immunofluorescent staining with anti-CTCF rabbit polyclonal antibody (left panel) and anti-B23 antibody (right panel) in differentiated neurons from rat trigeminal ganglia. Bar, 10 µm. (B) Immunoelectron localization of CTCF in the nucleolus of trigeminal ganglion neurons. Note the typical reticulated configuration of the nucleolus with its granular component (GC), dense fibrillar component (DFC) and fibrillar centres (FC). Gold particles indicating CTCF immunoreactivity preferentially decorated the dense fibrillar component of the nucleolus of rat neurons. Bar, 200 nm.





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