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Figure 6


Fig. 6. Nucleolar localization of the zinc-finger region of CTCF is independent of the transcription from rDNA. UR61 cells were transfected with the construct harbouring the central zinc-finger domain of CTCF fused with the GFP (GFP-ZF) and either left untreated (A) or treated 24 hours post-transfection with 0.05 µg/ml actinomycin D (ActD) (B) for 1 hour to specifically inhibit RNA pol I. Cells were fixed and immunostained for UBF, fibrillarin or B23 as indicated. Nucleic acids were visualized with propidium iodide (PI). The localization of the GFP-ZF fusion protein (a,d,g,j; green channel), the markers for the different nucleolar compartments (b,e,h,k; red channel) and the merged images (c,f,i,l) are shown. Confocal images show that GFP-ZF remains in the nucleolus following the inhibition of RNA pol I transcription. Bars, 10 µm.





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