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Figure 7


Fig. 7. Full-length CTCF, but not CTCF-ZF localization in the nucleolus requires protein synthesis. UR61 cells were transfected with the GFP-CTCF full length (GFP-CTCF; a,b,e,f) or with the GFP-CTCF-zinc-finger (GFP-ZF; c,d,g,h). 24 hours post-transfection cells were treated with 20 µg/ml cycloheximide (Chx) for 3 hours to inhibit protein synthesis. Untreated cells served as control. Cells were then fixed and analyzed by confocal microscopy for green fluorescence (a-d). Nucleoli were stained with propidium iodide (e-h). GFP-CTCF fusion protein, but not GFP-ZF, is delocalized from the nucleolus upon protein synthesis inhibition with cycloheximide. Bar, 5 µm.





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