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Figure 2


Fig. 2. Representative images of fluorescence of the Fluo-3 Ca2+-indicator in cultured rat aortic smooth muscle cells. Images were captured every 15 seconds to avoid photo-bleaching. Baseline fluorescence was recorded before treatment with ET-1 (-10 seconds). ET-1 was added at 0 seconds and the first image was captured at 5 seconds. Although the cell boundary was not clear, a transient increase in fluorescence intensity was seen in almost all cells.





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