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Fig. 3. Representative traces of fluorescence of the Ca²⁺-indicator Fluo-3 in cultured VSMCs after various treatments. (A-F) Cells were serum-starved, loaded with Fluo-3 (see Materials and Methods for details), and then subjected to various agonists (1 µM ATII, 0.1 µM ET-1, or 100 µM phenylephrine (PE). (B,C) Average time courses of changes in [Ca²⁺]_i in cells stimulated with (B) ATII and (C) ET-1 (n=3). Dotted lines in A-C show the equivalent levels of [Ca²⁺]_i before stimulation. (D) PE-induced decrease in [Ca²⁺]_i, although ET-1 still increased [Ca²⁺]_i in the presence of PE. (E,F) Cells treated with (E) Ca²⁺-free solution or (F) of the Ins(1,4,5)P₃-receptor antagonist 2-APB m (90 µM) for 10 minutes. Traces are representative of three to four independent experiments for each condition.

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