|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. Representative traces of contractile responses to various agonists in (A,B) reconstituted rat aorta VSMC fibers and (C,D) fresh tissues. Reconstituted VSMC fibers were cultured in collagen 3D-matrix in FBS-supplemented medium for 2 weeks and in the serum-free culture medium for an additional day (see Materials and Methods). (A) Contractions were induced with high-K+ solution (124 mM), ATP (200 µM), serotonin (5-HT, 10 µM), ATII (1 µM), PDBu (1 µM) or ET-1 (1 µM). (B) Phenylephrine (PE, 100 µM) and noradrenaline (NA, 10 µM) both induced relaxation, which was blocked by 30 µM propranolol. (C) Contractions of the fresh rat aorta tissues were induced by high-K+ solution, ATP, 5-HT, ATII, or ET-1 at the concentrations given for A. (D) A small contraction induced by 100 µM PE was enhanced by the presence of 30 µM propranolol. Force traces are representative of at least four (in A-C) and three (in D) similar experiments.
|
