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Figure 6


Fig. 6. Effect on phosphorylation of MYPT1 at (A) Thr696 and (B) Thr853, and of (C) CPI-17 at Thr38 after pretreatment with inhibitors. Cells were pretreated for 30 minutes with Y-27632 (30 µM) before addition of either ATII (1 µM) or ET-1 (0.1 µM) for 2.5 minutes. ET-1-treated samples were normalized to 1 for (A) MYPT1 Thr696, (B) MYPT1 Thr853 and (C) CPI-17 Thr38. *, significantly different to normal agonist treatments (n=3-4).





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