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Figure 2


Fig. 2. Effects of PCLN-1 expression on paracellular permeability. (A) Mock (open columns) and FLAG-tagged PCLN-1-expressing MDCK cells (closed columns) were plated on Snapwell polyester filters. TER values were measured at 7 days after plating. (B,C) Mock (open columns) and FLAG-tagged PCLN-1-expressing cells (closed columns) were cultured for 7 days. The absolute permeability for Na+ (PNa) and Cl- (PCl) and relative permeability of Na+ to Cl- (PNa/PCl) were calculated as described in the Materials and Methods. **P<0.01, compared with mock cells. NS, not significant compared with mock cells.





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