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Figure 5


Fig. 5. Localization of PCLN-1 in the TJ. FLAG-tagged PCLN-1-expressing MDCK cells were treated with 50 µM H-89, 10 µM PKI, 50 µM DDA or 50 µM DDA for 1 hour. When used, 100 µM 8-Br-cAMP (cAMP) was treated for 1 hour after the addition of DDA. Cells were double stained with anti-FLAG antibody (green) and anti-ZO-1 antibody (red). The x-y sections represent the area of the TJ. Right panels (x-z) show the vertical sections indicated by the triangles at the merged images. Bar, 10 µm.





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