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Figure 7


Fig. 7. Lysosomal targeting of PCLN-1 by dephosphorylation. (A,B) MDCK cells expressing WT PLCN-1 were grown to a confluent condition on cover glasses. Cells were incubated in the absence (A) and presence (B) of 50 µM H-89 for 1 hour. The cells were then double stained with anti-FLAG antibody (green) and organelle markers (red) of lysosomes (lysotracker), Golgi (furin) or early endosomes (EE, early endosomal antigen 1). (C) MDCK cells expressing the S217A mutant PCLN-1 were double stained with anti-FLAG antibody (green) and ZO-1 (red) or organelle markers (red) of lysosomes, Golgi or EE. The x-y sections represent the area of the TJ. Bar, 10 µm.





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