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Figure 2


Fig. 2. Comparison of PRL-cleaving activity of lysates from rat and human chondrocytes and other human cells. (A,B) Reducing western blot analysis of the PRL proteolytic products obtained at pH 7 when 200 ng of rat or human PRL were incubated in the absence (-) or presence (+) of 2 µg of protein from lysates of rat chondrocytes (A), or when 200 ng of human PRL were incubated with the indicated protein concentrations of lysates from human or rat chondrocytes (B). Arrow indicates the 16K-PRL proteolytic product. (C) The PRL (23K-PRL) and 16K-PRL bands obtained after incubation with various protein concentrations of lysates from human or rat chondrocytes were evaluated densitometrically and the results are expressed as a percentage of the 23K-PRL band evaluated after incubation in the absence of chondrocytes. (D) Reducing western blot analysis of proteolytic cleavage products resulting from the incubation of 200 ng of human PRL with increasing concentrations of lysates from human chondrocytes, skin fibroblasts, U937 lymphoma cells, and A549 lung carcinoma cells. Positions of the PRL isoforms of the indicated relative molecular mass are shown on the left of western blots.





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