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Figure 1


Fig. 1. A subpopulation of radially directed MTs extend into E-cadherin-based cell-cell contacts. MCF-7 cell monolayers were fixed at confluence and processed for epi-illumination immunofluorescence microscopy or electron microscopy. (A-D) Dual-label immunofluorescence imaging for E-cadherin (A) or ß-tubulin (B). A subpopulation of MTs appeared to radiate from the perinuclear area into sites of cadherin-rich cell-cell contacts (C,D); D shows the magnified boxed region in panel C. Distal tips of these MTs often appeared to terminate in concentrations (`puncta') of E-cadherin staining (D, arrowhead). Bar, 5 µM in panel D. (E-I). Gallery of electron microscopic images of junctional areas in MCF-7 cells and in hE-CHO cells. MTs (arrowheads in E), recognised by their characteristic morphology, run close to the adherens junction (between arrows), in some cases entering the dense microfilament network underlying the junctional complex. Panels F-I show lower magnification views of junctional areas in which MTs have been traced by hand and coloured to facilitate their recognition (E and F show the same image, F with MTs traced). Bars, 200 nm (F-I same magnification).





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