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Figure 3


Fig. 3. E-Cadherin homophilic ligation is sufficient to recruit MTs into adhesion sites. CHO cells stably expressing full-length human E-cadherin (hE-CHO) and E-cadherin lacking the p120 binding site (764-CHO) were grown to 80% confluency on glass coverslips. Latex beads (6 mm diameter) coated with either hE/Fc or ConA were allowed to adhere to the dorsal surfaces of cells for 90 minutes. Samples were immunolabelled for E-cadherin and tubulin, and visualised by laser scanning confocal microscopy. (A) Adhesion of hE/Fc-coated beads, but not ConA beads (asterisks), was associated with the accumulation of E-cadherin around the beads and projection of MTs into the sites of adhesion. (B) MT recruitment was quantified by counting the number of individual MTs that extended into contacts made between hE/Fc or ConA-coated beads and cells expressing full-length E-cadherin (hE-CHO) or CHO cells expressing the hE-Cad 764AAA mutant (764-CHO). We included all MTs that appeared to come into contact with the beads. Data are means ± s.e.m. (n=30 beads) and are representative of three separate experiments.





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