spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. ZR75 cells were stably transfected with the plasmids pLacIOP (-) or pLacIOP-caveolin-1 (+). (A) Survivin and caveolin-1 expression was assessed by western blotting. A representative result is shown. Residual survivin protein levels were 0.53±0.09 as determined in three independent experiments (*P<0.05). (B) Survivin mRNA content was analyzed by RT-PCR. Residual survivin mRNA levels were 0.50±0.06 as averaged from three independent experiments (*P<0.05). (C) Cell proliferation was measured by the MTS® assay in the presence and absence of caveolin-1. (D) Apoptosis was determined as indicated above by PI staining followed by flow cytometry. Numerical results are mean ± s.e.m. from three independent experiments; # and § comparisons with the mock control pLacIOP, #P<0.01, §P<0.05.





Right arrow Return to article