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Figure 1


Fig. 1. Pax7 remains able to drive transcription in some satellite cell progeny. Co-immunostaining of freshly isolated (T0) EDL myofibres derived from P34 mice, that report Pax3/Pax7 transcriptional activity, demonstrate that ß-gal protein (a, arrows) is present in quiescent satellite cells, as shown by the presence of Pax7 protein (b, arrows). After 40 hours (T40) in culture, ß-gal levels are variable in proliferating MyoD+ satellite cells, even within the same cluster (c and d, arrows). Clear divergence in the fate of satellite-cell progeny is evident by ~3 days (T67) in culture, with most cells downregulating Pax7 and committing to differentiation whereas others maintain Pax7 and lose MyoD. At this time, there are few satellite cell progeny with ß-gal activity after incubation in X-gal (e) and immunostaining shows these cells contain Pax7 (f-h, arrows). ß-gal levels are low/absent in cells committed to differentiation (i-l) as shown by the presence of MyoD (j, arrows) and myogenin (l, arrows). Counterstaining with DAPI was used to identify all nuclei present. Bar, 30 µm.





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