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Figure 1


Fig. 1. Western blotting of secretory alkaline phosphatase (SEAP) expressed in INS-1 ß-cells. (A) A stably transfected clone of INS-1 cells (clone 12, Trnfxn) immunoblotted for alkaline phosphatase next to untransfected control cells (Con). In addition, a faster-migrating specific band is detected in the stably transfected cells. Upon glycan digestion (+), the steady state distribution of SEAP is divided into endo-H-sensitive (S) and endo-H-resistant (R) forms, the latter indicative of a significant Golgi and/or post-Golgi pool of the protein in these cells. (B) A comparison of SEAP expression level in the clone shown in panel A (C-12) to two other independently selected INS-1 clones (C-14 and C-15), each of which maintains a similar proportion of endo-H-resistant SEAP.





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