spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. (A) Purified recombinant TbNRKC-6His protein (NRKC, black left arrowhead) phosphorylates {alpha}/ß casein substrate in vitro and preferentially phosphorylates ß casein (lane 2 arrowhead). The negative control BSA (apparent molecular mass 67 kDa) is not phosphorylated (lane 3). Purified kinase-dead TbNRKC-6His protein (mNRKC) (red arrowhead) does not phosphorylate the {alpha}/ß casein (lane 7) or BSA (lane 8). Controls: lane 1 TbNRKC alone, lane 4 {alpha}/ß casein alone, lane 5 BSA alone, lane 6 mNRKC alone. (B) Corresponding Coomassie-Blue-stained SDS-PAGE as protein loading control. (C) Corresponding western blot with Isa-1 polyclonal antibody as recombinant protein loading control. Pre-stained (left) and non-prestained (right) molecular mass markers show a slight discrepancy in the relative migration in the gel. Note that the recombinant mNRKC protein (B, position indicated by red arrowhead) migrates at a lower molecular mass than the recombinant NRKC (B, position indicated by black arrowhead). Arrowheads indicate where mNRKC and NRKC would normally run on SDS-PAGE.





Right arrow Return to article