(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 1. Interaction of AMPK 
1 subunit with plectin. (A) Schematic representation showing the domain structure of plectin and the fragment (ple-R5-6; I4218-G4543; GenBank accession number AY480038) used as bait in the yeast two-hybrid screening (Osmanagic-Myers and Wiche, 2004). The actin- and IF-binding domains (ABD, IFBD), the rod and C-terminal repeat domains 1-6 of plectin are indicated. The isolated AMPK 1 subunit clone contained the entire coding sequence comprising four CBS domains. (B) Plectin was immunoprecipitated from lysates of wild-type (+/+) myotubes using anti-plectin antiserum. Lysates (Lys) and immunoprecipitates (IP) were analysed by immunoblotting using antibodies immunoreactive with AMPK 1 subunit and plectin. Note Co-IP of AMPK 1, and absence of signal in control sample (C) run without precipitating antibodies and in sample from plectin-deficient myocytes (-/-). (C) Immunoprecipitation of 
1, 2, and 1 AMPK subunits from lysates of differentiated wild-type myocytes (myotubes) using indicated antibodies, and detection of cosedimenting 1 and 1 AMPK subunits by immunoblotting.
