spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Differentiation-dependent and subunit-dependent interaction of plectin with AMPK. (A,B) Lysates prepared from wild-type (+/+) and plectin deficient (-/-) undifferentiated myoblasts (A), or differentiated (6 days) myotubes (B) were incubated with antiserum #46 to plectin (IP), or without antibodies (C) for 3 hours. Immunocomplexes formed were isolated using protein G beads, separated by 10% SDS-PAGE and subjected to immunoblotting using antiserum to plectin, a 1:1 mixture of antibodies to the {alpha}1 and {alpha}2 subunits of AMPK (total AMPK), or antibodies recognising AMPK {alpha}1 or {alpha}2 subunits individually. AMPK {alpha}1 was co-precipitated with plectin only from lysates prepared from differentiated myotubes; AMPK {alpha}2 did not co-precipitate with plectin at any stage. (C) Lysates prepared from myotubes as in B were incubated with antibodies to AMPK {alpha}1 or {alpha}2 and immunoprecipitates were analysed by immunoblotting using the antibodies indicated. Lys, immunoblots of lysates.





Right arrow Return to article