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Figure 5


Fig. 5. Plectin deficiency alters the staining pattern of AMPK catalytic subunits {alpha}1 in skeletal muscle fibres. Frozen tissue sections of m. soleus isolated from 3-month-old wild-type (A-E,K-O; ple +/+) and muscle-specific plectin knockout (F-J,P-T; ple -/-) mice were immunolabelled using antibodies to AMPK {alpha}1 (A,F) and {alpha}2 (K,P) subunits, and plectin (B,G,L,Q). Note that plectin-specific staining in knockout tissue is restricted to connective tissue and blood vessels; only AMPK {alpha}1, but not {alpha}2, displayed extensive colocalisation with plectin (compare magnified boxed areas E and O); and fibres without plectin showed reduced AMPK {alpha}1 staining, and the striated staining pattern of the {alpha}1, rather than the {alpha}2 AMPK subunit was considerably deteriorated compared with wild-type (magnified boxed areas D, I and N, S, respectively). Bar, 10 µm.





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