spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. The {alpha}2ß1 integrin plays a role in the distribution of focal adhesions in fibroblasts. Wild type (WT) and {alpha}2-deficient ({alpha}2-/-) fibroblasts were seeded on glass coverslips coated with collagen I (40 µg/ml). After 60 minutes of adhesion, adherent cells were fixed and double-stained for fibrillar actin (A,D) and vinculin (B,E). The cells were observed by confocal microscopy and images were captured using single channel excitation. The two images were superimposed (C,F) using Photoshop (Adobe). Numerous vinculin-positive focal adhesions are present on the entire basal surface of {alpha}2-deficient fibroblasts, but not of wild-type fibroblasts (asterisk). Inserts in C and F represent higher magnifications of focal adhesions at actin stress fibre termini (arrows).





Right arrow Return to article