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Fig. 1. MMP13 and iNOS protein levels during MC3T3-E1 differentiation. MC3T3-E1 cells were analyzed at the time points indicated in the presence of 50 µg/ml ascorbic acid and 10 mM ß-glycerol phosphate. MMP-13 (upper panel) and iNOS (lower panel) were detected by immunoblot (n=3), whereas NO production was detected by Griess reaction (lower graph) (n=3).