spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Identification of {alpha}ENaC in human endothelial cells (HUVEC; representative experiment). Membrane proteins were separated by SDS-PAGE and stained with Coomassie brilliant blue (lane 1). {alpha}ENaC was detected by using an anti-{alpha}ENaC antibody (lanes 2-4). The amount of {alpha}ENaC in the aldosterone-treated sample (A) is about twice that in the control (C) and about 6 times higher than that of aldosterone and spironolactone (A+S)-incubated HUVEC. As positive control (PC), we used ENaC-expressing oocytes. The molecular mass standard is given on the left. The upper band in the range of 90 kDa most likely represents a glycosylated form of ENaC.





Right arrow Return to article