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Figure 1


Fig. 1. FMRP and FXR1P are co-translationally methylated by an endogenous MT in RRL. (Top panels) FMRP or (middle panels) FXR1P was produced in RRL in the presence of [3H]SAM and in the presence (lane 3) or absence (lane 2) of 6 µM AdOx, as indicated. Aliquots (20 µl) were resolved by SDS-PAGE and subjected to fluorography, while 5 µl aliquots were blotted and probed with anti-FMRP mAb or anti-FXR1P pAb. Lane 1 shows background methylation in the absence of the plasmids used in the lanes 2 and 3 translations. (Lower panels) Purified histone H3 (2 µg) from calf thymus was incubated with RRL in the presence of [3H]SAM and in the presence (lane 3) or absence (lane 2) of 6 µM AdOx. Aliquots (10 µl) were resolved by SDS-PAGE and subject to fluorography, while 10 µl aliquots were subjected to Coomassie Blue staining.





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