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Figure 3


Fig. 3. Intracellular distribution of L-plastin phosphorylation variants in Vero cells. (A,B) Transfected Vero cells expressing wild-type L-plastin (upper rows), L-plastin Ser5Ala (middle rows) or L-plastin Ser5Glu (lower rows) were processed for immunofluorescence double-staining. (A) Co-distribution of L-plastin variants with F-actin. Cells were stained with Rhodamine-phalloidin (left column) and anti-sv-tag antibody (middle column). Secondary antibody as in Fig. 2A. Merged images of enlarged regions (boxes) of left and middle images are shown on the right. Red, F-actin; green, total L-plastin. Bars, 15 µm. (B) Co-distribution of L-plastin variants with vinculin. Cells were double-stained with vinculin (left column) and sv-tag antibody (middle column). Secondary antibodies as in Fig. 2A. Merged images of enlarged regions (boxes) of left and middle images are shown on the right. Red, vinculin; green, total L-plastin. Bars, 15 µm.





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