Supplemental Figure 1
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Fig. S1. Removal
of the epitope tag from myc-AtPex11a, -d and -e does not alter
induced morphological and/or numerical changes in Arabidopsis peroxisomes at 24 or 48 hours. Arabidopsis suspension cells were co-transformed (biolistic
bombardment) with cytosolic GFP (A-L) and AtPex11a (A-D), AtPex11d (E-H), or AtPex11e
(I-L). Following bombardments (24 or 48 hours; bottom labels), cells were fixed
in formaldehyde, cell walls perforated/digested with pectolyase, and membranes
permeabilized in Triton X-100. Transformed cells were identified on microscope
slides through GFP autofluorescence (A,C,E,G,I,K) or anti-catalase/RhodamineX
fluorescence (B,D,F,H,J,L) (1:2000; 1 hour each). Peroxisomes bearing AtPex11a were elongated at 24 hours (B) and rounded and
increased in number at 48 hours (D). Peroxisomes bearing AtPex11d were elongated at 24 hours (F) and 48 hours
(H). Peroxisomes bearing AtPex11e
were spherical/rod-shaped at 24 hours (J) and rounded/proliferated at 48 hours
(L). Comparisons with myc-tagged versions of each protein (Figs 4 and 5) show
that removal of the epitope tag did not influence induced peroxisomal
morphologies and/or number per cell. Bar, 10 μm.
